lysate

The lysate is then filtered and placed on the ion-exchange column.

Archive 2005-10-01 2005

( "lysate") and GST-Plk1-PBD associated 53BP1 were analyzed by immunoblotting using anti-GFP antibody.

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Most striking was the finding that depletion of haem, and addition of GSSG or dsRNA all seemed to have similar effects on cell-free protein synthesis in the reticulocyte lysate, as though these disparate agents all acted in a similar manner.

Tim Hunt - Autobiography 2002

One other thing I learned about during the studies on the inhibition of protein synthesis by dsRNA was that micrococcal nuclease, which requires Ca2+ for activity, did not inhibit protein synthesis in the reticulocyte lysate provided no Ca2+ was present.

Tim Hunt - Autobiography 2002

I also discovered that simple gel-filtration of the lysate caused a drastic fall in its activity.

Tim Hunt - Autobiography 2002

By 1977, the outlines of all this were pretty clear, and we had a very good understanding of the reticulocyte lysate and its properties.

Tim Hunt - Autobiography 2002

The micrococcal nuclease calcium dependence was put to good use by Hugh Pelham, a graduate student in the lab, who developed the use of nuclease treatment of the lysate to assay heterologous mRNAs.

Tim Hunt - Autobiography 2002

In the left panel, 3% of the lysate input used in the immunoprecipitations was subjected to western blot analysis using an anti-ubiquitin antiserum to show that general ubiquitination levels were similar in all genotypes.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

Plasma membrane and total cell lysate proteins were resolved by SDS-PAGE, blotted on PVDF membrane and probed with anti-GLUT4 antibody.

PLoS ONE Alerts: New Articles Alfredo Csibi et al. 2010

In the left panel, 3% of the lysate input used in the immunoprecipitations was subjected to western blot analysis using an anti-ubiquitin antiserum to show that general ubiquitination levels were similar in all genotypes.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010