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- n. Proper modern spelling of homogenizer outside North America.
Sorry, no etymologies found.
I dissociated the lymphocytes with a Dounce glass homogeniser and started their stimulation in culture with a bacterial mitogen, Protein A, known as an activator of B and T lymphocytes, since I did not know which fraction of lymphocytes could produce the putative virus.
The cells were then lysed with 30 strokes in a tight - fitting glass dounce homogeniser in lysis buffer (250 mM sucrose, 50 mM Tris-HCl pH 7.6, 1 mM MgCl, 1 mM DTT, 1 mM PMSF).
Buffer A (250 mM sucrose, 50 mM Tris-HCl (pH 7.4), 1 mM PMSF, 20 µg/ml aprotinin) was added to further help solubilisation and a further 20 strokes applied in the dounce homogeniser.
AEL and Lake break new ground with bulk emulsion explosives homogeniser