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- abbreviation organic chemistry
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* P Frequency of BrdU+ cells in the indicated BM B cell developmental subsets from mice treated with saline or FTY720 (1 mg/Kg) for 3 days, and continuously exposed to BrdU in the last 24 h.
(400-600X) magnification (Zeiss AxioHOME microscope) and crypt epithelial cells (paneth and non-paneth) intestinal sections were examined using ImageJ software and classified as BrdU positive if they grossly demonstrated brown-stained nuclei from DAB staining or as BrdU negative if they were blue stained nuclei.
(C) BrdU incorporation for 48 h in developing B cell subsets from mice treated with saline (white bars) or FTY720 (1 mg/Kg; gray bars) for 3 days.
(A) BrdU incorporation assays revealed a higher proliferation rate in primary calvarial osteoblast cultures from transgenic mice after 2 days of differentiation.
(B) BrdU incorporation for 48 h in developing B cell subsets from
Pre-B cells became similarly BrdU labeled in the two groups indicating that S1P1-signaling is not required for Pro-B and Pre-B cell expansion or survival.
BrdU incorporation assays were performed at day 2 of differentiation using the Cell Proliferation Biotrak ELISA (GE Healthcare, #RON250), while extracellular matrix mineralization was assessed at day 10 of differentiation using von Kossa-staining
In FTY720, and BrdU treatments, in vivo labeling was performed in the last 2 minutes of the indicated treatments.
Pomc (b) or Npy (c) in conjunction with an antibody against BrdU and counterstained for DAPI.
Our finding of a reduction in 48 hr BrdU-labeled S1P1 - and S1P-deficient immature-BM B cells compared to wildtype controls indicated that it takes longer to fully replace S1P1 - and S1P-deficient immature cells with cells newly generated from pre-B cells.
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