DNAase

Recombinant latherin was further purified by gel filtration (Superdex 75, 16 / 60, 50 mM Tris, 0.25 M NaCl, pH 8.4; Amersham Pharmacia / GE Bioscience, Chalfont St Giles, Buckinghamshire, UK). mRNA was extracted from equine tissues using Trizol treated with DNAase I (Ambion) to remove genomic material. cDNA was synthesised using Superscript II reverse transcriptase

PLoS ONE Alerts: New Articles Rhona E. McDonald et al. 2009

More recently, clinical Chinese hamster ovary cells containing DNA trials on this agent have been conducted evaluating encoding for the native human protein, deoxyribo - its effects on other putatively B lymphocyte - nuclease I (DNAase), was essentially irrelevant to mediated diseases such as rheumatoid arthritis. the design of the clinical development program.

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First-strand cDNA was synthesized from 2 µg of DNAase I-treated total RNA with random hexamer primers using TaqMan Reverse Transcription Reagents (N808-0234, Applied Biosystems).

PLoS ONE Alerts: New Articles Li Zhou et al. 2009

Briefly, tissue was collected and frozen in liquid nitrogen, pulverized, and then RNA was extracted using RNAeasy with DNAase on column treatment (QIAGEN), and labeled and hybridized to Affymetrix ATH1

PLoS Biology: New Articles 2008

Equal amounts of RNA were treated with RNAase-free DNAase I (Ambion Inc.).

PLoS ONE Alerts: New Articles Li Zhou et al. 2009