Sepharose

Recombinant proteins were purified by affinity chromatography using Glutathione Sepharose 4B (Amersham).

PLoS ONE Alerts: New Articles Chang-Jin Park et al. 2010

Intact GST-fusion proteins were incubated with glutathione Sepharose 4B beads (GE Healthcare) for 2 h.

PLoS Biology: New Articles Dimple Notani et al. 2010

His6-pirin was incubated with glutathione-Sepharose beads that were conjugated to GST or GST-Bcl3 ARD.

Naturejobs - All Jobs Isao Miyazaki 2010

Recombinant proteins were purified by affinity chromatography using Glutathione Sepharose 4B (Amersham).

PLoS ONE Alerts: New Articles Chang-Jin Park et al. 2010

Protein G-Sepharose beads were blocked in 0.1% BSA in binding buffer for 2 hours.

PLoS ONE Alerts: New Articles Parveen Sharma et al. 2010

Clarified cell lysate was loaded onto a Nickel Chelating Sepharose Fast Flow column (GE healthcare), the His-tagged SSADH protein was eluted in 150 mM NaCl, 250 mM imidazole, 5 mM β-mercaptoethanol, 50 mM Tris (pH 8.0).

PLoS ONE Alerts: New Articles Christopher G. Langendorf et al. 2010

The lysate was then incubated with either mouse anti-V5 antibody (2 µg) or equal amount of non-specific mouse IgG for 2 h before pulldown with rec-protein-G-Sepharose

PLoS Biology: New Articles Ting-Ting Hong et al. 2010

Human seminal plasma from subjects with normal semen parameters were separated on a gelatin-Sepharose column and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting using antibodies against distinct FN forms.

Naturejobs - All Jobs Maja M. Kosanović 2010

Protein G Sepharose (GE Healthcare, Piscataway, NJ) beads were then added to the lysate for 3 hours, collected by low-speed centrifugation, washed three times in lysis buffer and boiled in 2X SDS-PAGE sample buffer with 2% beta-mercaptoethanol for 10 minutes to release immunoprecipitated dMfn.

PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010

After purification with an Ni Sepharose ™ 6 Fast Flow (GE Healthcare), rSeTre-1 and rSeTre-2 were used to immunize rabbits as described previously

PLoS ONE Alerts: New Articles Jie Chen et al. 2010