Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun 5-bromo-4-chloro-3-
indolyl -β-D-galactopyranoside , used to detect β-galactosidase .
Etymologies
Sorry, no etymologies found.
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Examples
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From the colonies that appeared on the plates, a loopful of cells was taken and resuspended in Lemco broth, mixed with vortexing, serial dilutions were plated onto Lemco agar supplemented with 2% sucrose and 50 µg/ml X-gal and allowed to grow for two days.
PLoS ONE Alerts: New Articles Arunava Dasgupta et al. 2010
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(Invitrogen, Carlsbad, CA) and plated onto agar plates with X-gal and 150 mg/mL Carbenicillin.
PLoS ONE Alerts: New Articles Jenna L. Morgan et al. 2010
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Unfortunately, both heterozygotes and homozygotes carrying the lacZ allele did not express β-galactosidase protein or activity by immunofluorescence or X-gal staining, respectively (data not shown).
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This suggests that Muc16-lacZ chimeric transcripts may be very unstable, leading to insufficient production of β-galactosidase for detection by immunofluorescence and X-gal staining (data not shown).
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LacZ reporter: the qualitative X-gal colony-lift filter assay and quantitative
PLoS ONE Alerts: New Articles Hiu-Kwan Chow et al. 2008
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LacZ reporter: X-gal colony-lift filter assay and ortho
PLoS ONE Alerts: New Articles Hiu-Kwan Chow et al. 2008
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Colonies were plated on LB Kanamycin with X-gal and grown overnight.
PLoS ONE Alerts: New Articles Joseph L. Mankowski et al. 2008
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In the X-gal colony-lift filter assay, the lysed yeast cells were incubated with X-gal for three hours, and blue color developing after three-hour incubation was not considered to be indicative of positive protein: DNA interactions.
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LacZ reporter: X-gal colony-lift filter assay and ortho
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h and then plated on Lemco agar supplemented with hygromycin B, kanamycin and 50 µg/ml X-gal.
PLoS ONE Alerts: New Articles Arunava Dasgupta et al. 2010
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