bafilomycin

Since bafilomycin A1 inhibits the degradation of autophagosomes, the levels of LC3-II in bafilomycin A1-treated cells reflect the rate of autophagosome formation

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

Upon treatment of the lysosomal inhibitor bafilomycin A1, which prevents the autophagic degradation of p62 and LC3-II, both p62 and

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

The difference in p62 level between cells treated with bafilomycin A1 and untreated cells represents the amount of p62 that has been recruited into autophagic vesicles during the period of treatment.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

The cells were then washed with PBS for three times and then starved in Earle's balanced salt solution (EBSS) or EBSS with bafilomycin A1 (BafA) (0.1 µM) in 37°C for 2 hr before harvesting for Western blot analysis.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

CHM and control monocytes were pretreated with 100 nM bafilomycin A1 for 30 min before the feeding.

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009

FACS analysis of the monocyte population from CHM patients (n = 3) and controls (n = 3) at 1, 3 and 5 h following the treatment with bafilomycin A1 demonstrated a decrease in the levels of fluorescent intensity of engulfed pH-dependent BioParticles in both controls and patients.

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009

To confirm that the increase in lysosomal pH affected the ability of monocytes to degrade engulfed material, cells from controls and CHM patients were treated with the vH+ATPase inhibitor, bafilomycin A1.

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009

The fluorescence level for a selected patient before and after bafilomycin A1 treatment is shown in

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009

To measure the effect of proton pump inhibitors on phagocytosis in CHM monocytes and fibroblasts compared to the controls, cells were pretreated for 30 min with 10 µM of bafilomycin A1 before the phagocytic assay.

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009

A number of studies have demonstrated that bafilomycin A1 causes an increase in pH inside the lysosomes, leading to a decrease in the rate of degradation of material in lysosomes

PLoS ONE Alerts: New Articles Natalia V. Strunnikova et al. 2009