bromophenol

The positions of the xylene cyanol (XC) and bromophenol blue (BPB) marker dyes are indicated. doi: 10.1371/journal. pone.0009627.g003

PLoS ONE Alerts: New Articles Michel V. L�vesque et al. 2010

The mixture was precipitated with ethanol and resuspended with 5 µl of formamide-dye (0. 3% xylene cyanol, 0. 3% bromophenol blue, and 12 mM EDTA dissolved in formamide).

PLoS ONE Alerts: New Articles 2010

The mixture was precipitated with ethanol and resuspended with 5 µl of formamide-dye (0. 3% xylene cyanol, 0. 3% bromophenol blue, and 12 mM EDTA dissolved in formamide).

PLoS ONE Alerts: New Articles Michiko M. Nakano et al. 2010

The resulting pellets were dissolved in equal volumes of ultrapure water and loading buffer (95% formamide, 10 mM EDTA, pH 8.0, 0. 025% xylene cyanol and 0. 025% bromophenol blue).

PLoS ONE Alerts: New Articles Michel V. L�vesque et al. 2010

After completion of IEF, IPG strips were incubated for 15 min in equilibration solution I (50 mM Tris pH 6.8, 6 M urea, 30% glycerol, 2% SDS, 2% DTT, trace bromophenol blue) and 15 min in equilibration solution II (solution I with 2. 5% iodoacetamide instead of DTT).

PLoS ONE Alerts: New Articles Zhemin Zhou et al. 2010

Samples were loaded onto a native 8% polyacrylamide gel, and were electrophoresed at 100 V until the bromophenol blue dye had migrated approximately two-thirds to three-quarters of the length of the gel, and then the binding reactions were electro blotted onto nylon membrane at 380 mA (~100 V) for 1 h.

PLoS ONE Alerts: New Articles Shaohui Liu et al. 2010

We diluted the supernatants with SDS-PAGE loading buffer (100 mM Tris [pH 6.8], 20\% v / v glycerol, 4\% w / v SDS, 0. 02\% w / v bromophenol blue) with

PLoS Medicine: New Articles 2009

Thirty micrograms of each protein lysate were loaded in each lane in sample buffer (2\% SDS, 10\% glycerol, 0. 001\% bromophenol blue, 1\% DTT, and 0.05 M Tris-HCl, pH 6.8), separated on 10\% SDS-PAGE (Invitrogen), and transferred to a PVDF membrane (Millipore, Temecula, CA).

PLoS ONE Alerts: New Articles 2009

Methane monooxygenase 1.14.13.25 P bromophenol + NAD (P) + + H2 O

Recently Uploaded Slideshows arab2000.forumpro.fr@slideshare.net(arab2000.forum 2009

To terminate the reaction, 4× SDS sample buffer (125 mM Tris-HCl [pH 6.8], 6\% SDS, 20\% glycerol, 0. 02\% bromophenol blue supplemented with 10\% β-mercaptoethanol) was added, and the samples boiled for 5 min.

PLoS Biology: New Articles 2009