cRNA

The double stranded cDNA was then transcribed into biotin labelled cRNA using the Bioarray High Yield RNA Transcript Labelling Kit (Enzo Diagnostics, USA) according to the manufacturer's protocol.

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IVT reaction was performed overnight (~16 h) to yield sufficient biotinylated cRNA.

PLoS ONE Alerts: New Articles Ville Härmä et al. 2010

(Bio-Rad), 10 µg of fragmented cRNA samples was hybridized to a GeneChip® Mouse Genome 430 2.0

PLoS ONE Alerts: New Articles Atsushi Fukuda et al. 2010

Kit (Affymetrix) was used for synthesizing cRNA starting from 9 µl total RNA solutions.

PLoS ONE Alerts: New Articles Atsushi Fukuda et al. 2010

Short read sequencing was performed using total RNA including human RNA, and also vRNA, cRNA, and mRNA from influenza A virus; thus, coverage bias was detected throughout the segments, but the average coverage (AC) is likely to be similar at approximately ×200 or more.

PLoS ONE Alerts: New Articles Makoto Kuroda et al. 2010

RNA and cRNA concentrations were measured with a nanodrop ND-1000, overall quality was monitored with BioRad's Experion electrophoresis station. 750 ng cRNA were hybridized on Illumina's Sentrix HumanRef-8 v3

PLoS ONE Alerts: New Articles Ville Härmä et al. 2010

The nucleoprotein molecules are required for these two steps of replication and are deposited on the cRNA and vRNA during RNA synthesis

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Hybridized cRNA was detected with 1 mg/ml Cyanine3-streptavidine

PLoS ONE Alerts: New Articles Ville Härmä et al. 2010

Briefly, DNA templates for transcribing cRNA probes were generated by PCR.

PLoS ONE Alerts: New Articles Samir Koirala et al. 2010

The coverage is reflected by the amounts of vRNA, cRNA, and mRNA of influenza A virus, implying that the coverage bias may detect a more stable region as it is dependent on the expression level or manner of replication.

PLoS ONE Alerts: New Articles Makoto Kuroda et al. 2010