Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- verb Simple past tense and past participle of
cryoprotect . - adjective
protected fromcold by the action of acryoprotectant
Etymologies
Sorry, no etymologies found.
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Examples
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Brains were removed and cryoprotected by soaking in 30% sucrose solution in phosphate buffer until they sank.
PLoS ONE Alerts: New Articles Nadia G. Innamorato et al. 2010
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The crystals were cryoprotected in the mother liquor plus the addition of 15% glycerol and flash frozen in a nitrogen stream at − 180°C.
PLoS ONE Alerts: New Articles Michiko M. Nakano et al. 2010
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The images shown here were taken from cryoprotected cells, as the freezing quality was improved.
PLoS ONE Alerts: New Articles Coralie Spiegelhalter et al. 2010
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Brains were removed, post-fixed overnight in perfusant, then cryoprotected in a 30% sucrose solution.
PLoS ONE Alerts: New Articles Pramod K. Dash et al. 2010
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The crystals were cryoprotected in the mother liquor plus the addition of 15% glycerol and flash frozen in a nitrogen stream at − 180°C.
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Briefly, the fixed samples were cryoprotected with PVP/sucrose (20% polyvinyl pyrrolidone [Sigma] in 2.3 M sucrose) overnight and frozen in liquid nitrogen before being cut into thin sections with Leica Ultracut UCT with EMFCS attachment (Leica Microsystems Inc.).
PLoS Biology: New Articles Ting-Ting Hong et al. 2010
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Our results indicate that small numbers of organ cells had withstood and / or evaded cryoinjury from ice crystals and / or osmotic stress within the organ and that nuclei in the cells were intact after defrosting the non-cryoprotected frozen organs.
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There have been attempts to rescue animal genetic resources from dead cells or non-cryoprotected frozen specimens.
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Briefly, animals were perfused with 4\% paraformaldehyde, the brains removed from the skulls, cryoprotected and sectioned at 40 µm on a dry ice-cooled sliding microtome.
PLoS ONE Alerts: New Articles Alexander Garthe et al. 2009
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To our knowledge, this is the first report of the resurrection of a dead elite livestock specimen from a non-cryoprotected frozen organ by cloning.
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