Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biology A
steroid foundexclusively in theflowers andleaves of the plants Digitalispurpurea and Digitalislanata .
Etymologies
from Wiktionary, Creative Commons Attribution/Share-Alike License
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Examples
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Skerra 2000 A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin.
Flagellum evolution -- how's your German? - The Panda's Thumb 2010
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Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence.
PLoS ONE Alerts: New Articles Hortensia Sanchez-Calderon et al. 2010
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DNA probes were prepared by PCR using digoxigenin (DIG) - PCR labeling mix (Roche).
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For dual-color FISH with fluorescein - and digoxigenin-conjugated RNA probes, after the blocking step, embryos were incubated in POD-conjugated anti-fluorescein antibody (Roche) (1: 750) at RT overnight, followed by eight washes with MOPS wash buffer.
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Apoptosis was confirmed by DNA fragmenatation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay.
BioMed Central - Latest articles Gowhar Shafi 2009
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Total RNA was isolated from the wt, Δ pho4, or Δpho85 cells grown under high (H) - or low (L) - Pi conditions, subjected to northern blot analysis, and probed with a digoxigenin (DIG) - labeled probe for each gene as designated.
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For single-color fluorescent in situ hybridizations (FISH) using a digoxigenin-conjugated RNA probe and tyramide signal amplification (TSA-Plus kit; PerkinElmer), the above procedure was modified as follows.
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In Situ Cell Death Detection kit-AP (Roche, Germany) according to a previously described protocol Clones of the in situ markers were obtained from various labs (mentioned in the Acknowledgement section) and digoxigenin-labeled antisense RNA probes were generated with T7 or T3 RNA polymerases (Roche) according to the orientation of cDNA inserts.
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For immunohistological detection of digoxigenin (DIG), the sections were incubated overnight at room temperature with anti-DIG conjugated to alkaline phosphatase (Roche Diagnostic) diluted 1: 2000 in PBST containing 0. 2\% blocking agent (Roche Diagnostic).
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AS RNA, antisense RNA; ChIP, chromatin immunoprecipitation; DIG, digoxigenin; IP, inositol polyphosphate; ncRNA, noncoding RNA; Pi, inorganic phosphate
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