Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- adjective
purified byimmunopurification
Etymologies
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Examples
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The tryptic fragments were immunopurified with an anti-nitrotyrosine antibody and analyzed by MALDI-TOF mass spectrometry.
PLoS ONE Alerts: New Articles Alfredo Csibi et al. 2010
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Flag-histidine-tagged CrPV-1A was immunopurified with anti-Flag antibody and analyzed by western blotting with
Naturejobs - All Jobs Arabinda Nayak 2010
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An in vitro transcribed RNA was capped with guanylyltransferase using [α - 32P] GTP and then incubated with immunopurified intact gemin5 or the gemin5 (Δ25) deletion mutant in the presence or absence of free cap analog competitor.
PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009
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Crosslinking reactions (20 µl) contained 60 ng labeled RNA, 40 ng immunopurified protein or 120 µg cytoplasmic lysate, 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 1 mM MgCl
PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009
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RNA was isolated from extracts (input) and from the immunopurified
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Total RNA was isolated from cell extracts and immunopurified samples with the mirVana ™ PARIS ™ kit (Ambion).
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+ and Arg II+ MØ were identified with a purified rat anti-mouse Mac3 monoclonal antibody (BD Biosciences), an immunopurified rabbit anti-mouse Arg II polyclonal antibody
PLoS ONE Alerts: New Articles Jamila Khallou-Laschet et al. 2010
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[20], we tested whether human Tsc1 is also phosphorylated on Ser524, but could not detect any phosphorylation by mass spectroscopy with immunopurified hTsc1 from HEK293 cells (data not shown).
PLoS ONE Alerts: New Articles Sibylle Schleich et al. 2009
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(50-100 ng) of the immunopurified RNAs were used for amplification. 8 µg of the amplified RNAs
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Crucially, immunopurified gemin5 bound cap-sepharose in the absence of other proteins, and specifically crosslinked to radiolabeled cap-structure after UV irradiation (
PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009
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