microneme

We have shown that 4-BPB is a potent reagent that resolves two previously inseparable aspects of invasion, microneme and rhoptry secretion.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

Virtually nothing is known about the triggers and mechanism of rhoptry secretion or the means by which rhoptry and microneme proteins associate.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

This strongly argues that micronemes do not need to fuse to rhoptry necks to secrete and that formation of the MJ complex, a mix of microneme and rhoptry neck proteins, most likely occurs at the parasite surface.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

This suggests that the drug affected one or more parasite proteins involved in motility but unrelated to microneme secretion.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

This drug specifically blocked rhoptry secretion but not microneme secretion, thus effectively showing that the two processes can be de-coupled.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

To assay for the ability of microneme proteins to get to the parasite surface, parasites on coverslips coated with 5% FBS were fixed after 30 minutes.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

To determine if the inhibition of gliding motility might be due to a block in microneme secretion, we looked at the secretion of microneme protein 2 (MIC2) both constitutively and in the presence of 1% ethanol and ionophore A23187.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

This drug specifically blocked rhoptry secretion but not microneme secretion, thus effectively showing that the two processes can be de-coupled.

Elites TV 2009

Micronemes are known to be involved in parasite gliding motility, but microneme secretion was unaffected at drug concentrations that affected motility.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009

This provides new information about the initial secretion events: microneme proteins are able to be secreted onto the parasite surface despite the inhibition of rhoptry secretion.

PLoS ONE Alerts: New Articles Sandeep Ravindran et al. 2009