myofibers

The most prominent findings were scattered atrophic myofibers that were mostly type one oxidative phosphorylation dependent myofibers, mild increase in lipid in selected myofibers, and occasional myofiber with reduced cytochrome c oxidase activity.

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Green, GFP+ myofibers are observed in non-regenerating muscle as well, indicating that GFP+ melanoma cells fuse to pre-existing fibers.

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

Myogenic conversion of B16-GFP and LLC1-GFP cells was quantified by determining the number of green myotubes expressing either MyoD or MF20, and expressing this as a percentage of the total number of myotubes per field (% of GFP+ myofibers). 10 randomly chosen fields in 3 independent experiments were analyzed.

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

In the case of injured muscle, newly regenerated myofibers can be recognized by the presence of centrally located myonuclei

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

For detection of GFP+ myofibers, a narrow range of emission wavelength (511 to 532 nm) was used to avoid detection of autofluorescence Cells were collected and RNA was extracted using RNeasy minikit (Qiagen).

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

In addition, increased sarcolemmal permeability suggests functional impairment of the DGC complex in cardiac myofibers.

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In addition, increased sarcolemmal permeability suggests functional impairment of the DGC complex in cardiac myofibers.

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B16-GFP cells were able to fuse with the nascent or the pre-existing myofibers when directly or intra-arterially injected into injured or uninjured TA muscle.

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010

In addition, increased sarcolemmal permeability suggests functional impairment of the DGC complex in cardiac myofibers.

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Injection of GFP-labeled melanoma cells into uninjured or regenerating skeletal muscle results in GFP positive myofibers indicating that these cells are recruited into muscle fibers.

PLoS ONE Alerts: New Articles Ara Parlakian et al. 2010