Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biochemistry Several
histidine residues linked together (usually at least six)
Etymologies
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Examples
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Anti-polyhistidine peroxidase conjugate antibody (Sigma Aldrich) was used at 1: 10000 dilution.
PLoS ONE Alerts: New Articles Jean-Baptiste Rioux et al. 2010
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Anti-polyhistidine peroxidase conjugate antibody (Sigma Aldrich) was used at 1: 10000 dilution.
PLoS ONE Alerts: New Articles Jean-Baptiste Rioux et al. 2010
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Expression and purification of polyhistidine-tagged proteins
PLoS ONE Alerts: New Articles Zhihong Wu et al. 2009
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Antibody to polyhistidine (1: 1,000 dilution; Sigma) was used to distinguish exogenous proteins from endogenous proteins.
PLoS ONE Alerts: New Articles Wenjie Sun et al. 2009
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The expression of recombinant proteins was analyzed by electrophoresis on a 15% (wt/vol) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition and western-blotting with mouse antibody to polyhistidine (1: 1,000 dilution; Sigma) and sheep antibody to mouse Alkaline phosphatase (1: 10,000 dilution; Sigma).
PLoS ONE Alerts: New Articles Wenjie Sun et al. 2009
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Germany) and the polyhistidine-tagged protein was eluted with 1×elution buffer (50 mM NaH
PLoS ONE Alerts: New Articles Zhihong Wu et al. 2009
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Mouse monoclonal antibody to polyhistidine (1: 1,000 dilution;
PLoS ONE Alerts: New Articles Wenjie Sun et al. 2009
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