proinsulin

Unlike proinsulin which is virtually devoid of biologic activity

Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems 1977

These include the larger hormonal forms - proinsulin (19), big gastrin

Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems 1977

Our discovery of the immunochemical heterogeneity of parathyroid hormone (27) and Steiner's discovery of proinsulin (19) just over a decade ago initiated a revolution in concepts of biosynthesis of the peptide hormones.

Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems 1977

The newly discovered peptide eluted between insulin and proinsulin on Sephadex G50 gel filtration, in contrast with HG which eluted after insulin (Fig. 12).

Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems 1977

The original suggestion that a major function of proinsulin in biosynthesis was to facilitate disulfide bond formation

Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems 1977

Until now, there has not been a viable method for measuring rodent proinsulin, which would allow researchers to establish models for testing targeted therapies or interventions in the pre-clinical setting.

Press Releases 2010

While research has established that the total ratio of circulating proinsulin relative to insulin and c-peptide is altered in the diabetic and pre-diabetic state in humans, multiple barriers in the ability to accurately measure rodent proinsulin means relatively limited data has been available regarding concentrations of this molecule in rats and mice, both of which are widely used models in the diabetes research community.

Press Releases 2010

Several factors, not the least of which being the variability in expression of proinsulin I and II between mice and rats, makes the ALPCO species-specific assay kits the most attractive option for researchers expecting to quantify total proinsulin in their pre-clinical models.

Press Releases 2010

Yokota-Hashimoto H, Zhao S, Wang J, Halban PA, et al. (2003) Dominant negative pathogenesis by mutant proinsulin in the Akita diabetic mouse.

PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010

In cases of beta cell dysfunction, including type 2 diabetes and pre-diabetes, the proinsulin secretion ratio can increase to >

Press Releases 2010