Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun Plural form of
pseudovirion .
Etymologies
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Examples
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(A) HeLa cells were transfected with GFP-tagged dominant-negative inhibitors of clathrin-mediated endocytosis or a control (GFP-DIIIΔ2). 24 hours after transfection cells were exposed to AlexaFluor 546 labeled transferrin or to HPV16 pseudovirions for 10 hours and then fixed with methanol.
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The pseudovirions were purified from the cell lysates by Optiprep gradient centrifugation The HPV16 L1-specific antibodies mAb L1-7 (mouse monoclonal) and K75 (rabbit polyclonal) have been described previously
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Detection of extra - and intracellular pseudovirions.
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(D) siRNA transfected HeLa cells were exposed to pseudovirions for 10 hours and / or AlexaFluor 546 labeled transferrin.
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First, we tested whether entry of HPV16 pseudovirions into HeLa cells was affected by the expression of dominant-negative (dn) Eps15 mutants.
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(D) HeLa cells were infected with pseudovirions for 4 hours and then fixed with paraformaldehyde.
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Treatment of HeLa cells with HPV16 pseudovirions as in A. Cells were fixed with methanol, immunostained with a polyclonal anti-L1 antibody (K75, red) and a monoclonal anti-CD63 antibody (sc-5275) recognizing only internal CD63 under these conditions, and analyzed as in A.
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HeLa cells were exposed to HPV16 pseudovirions for the indicated time periods, and fixed with paraformaldehyde.
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(B) HeLa cells were exposed to HPV16 pseudovirions for the indicated time periods, and fixed with paraformaldehyde.
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(CtxB) for 30 min or exposed to HPV16 pseudovirions for 10 hours.
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