spoligotype

Overall, spoligotype patterns were obtained from 760 smears from 518 different patients, some with multiple episodes of disease. 153 patients had multiple results from the same episode of disease, from smears taken within 3 months of each other (75 pairs, 40 triplets, 12 sets of four, one set of 5 and one of 6).

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

Each spoligotype can have a range of RFLP fingerprints, so spoligotype clusters do not necessarily represent close epidemiological linkage, and increases in a particular spoligotype in a population are unlikely to result from single outbreak events.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

The increase in Lineage 3 was due to an increase in the family CAS1-Kili, and most (44/49) of the CAS1-Kili strains were a single spoligotype (ST21).

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

In order to define the lineage for the contact pairs we first linked the RFLP patterns to the spoligotype.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

Isolates were grouped into spoligotype families and lineages following previously published criteria.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

Assuming that other patients in the population with the same RFLP would have the same spoligotype, we inferred the spoligotype pattern for 100 of the RFLP-defined strains from the first cases in the pairs.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

Beijing strains had the identical, typical spoligotype.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

Trends over time for lineages, spoligotype families and the commoner spoligotypes.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

There was no evidence that the proportion of isolates that were isoniazid resistant varied by lineage, spoligotype or spoligotype family more than would be expected by chance.

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010

This may be an artefact, as the spoligotype that we have designated ST59c, which differs from 59 only in the absence of spacer 15 was found only up to

PLoS ONE Alerts: New Articles Judith R. Glynn et al. 2010