transfecting

Furthermore, scientists have been routinely transfecting (i.e., inserting) foreign pieces of DNA into all sorts of cells to confer on those recipient cells the abilities to make proteins that they otherwise would not make and to thereby influence, sometimes decisively, their functional properties more broadly.

Neil S. Greenspan: Genes that Promote Self-Promotion 2010

Colabelling is possible by transfecting cells with different plasmids each containing one fluorescent protein gene and one gene of interest (Figure 3).

Archive 2005-10-01 2005

It does no one any good to have someone with a degree in physics looking at a patent for a method of transfecting genes into plants.

Continuation Rules "Dead as a Doornail"? Peter Zura 2007

Pps were produced by transfecting 293T cells with four plasmids: an HA expression plasmid, an NA expression plasmid, a Gag-pol-encoding plasmid, and a reporter plasmid.

PLoS ONE Alerts: New Articles Yonghui Zhang et al. 2010

Subsequently, Yu et al. [8] obtained iPSCs in humans by transfecting with OCT4, SOX2, NANOG and LIN28.

PLoS ONE Alerts: New Articles Simone Pacini et al. 2010

Pps were produced by transfecting 293T cells with four plasmids: an HA expression plasmid, an NA expression plasmid, a Gag-pol-encoding plasmid, and a reporter plasmid.

PLoS ONE Alerts: New Articles Yonghui Zhang et al. 2010

Additional transactivation assays were carried out co-transfecting FOXL2 and increasing amounts of PIAS1 and UBC9 in COS-7 cells, to test whether FOXL2 transcriptional activity, like that of SOX9, is affected by PIAS1 in both SUMO-ligase-dependent and independent manners

PLoS ONE Alerts: New Articles Mara Marongiu et al. 2010

To evaluate whether down-regulation of bcl-2 shows opposite effect of bcl-2 overexpression in terms of HIF-1α protein expression, we silenced the endogenous expression of bcl-2 gene transfecting M14 cells with siRNA-targeting bcl-2 mRNA (si-bcl-2) and then exposing them to normoxia or hypoxia for 24 h.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Esau et al. [9] found that reducing miRNA-143 by transfecting antisense oligonucleotides inhibits adipocyte differentiation in vitro, suggesting that miRNAs may play a role in the regulation of key adipose tissue processes and could therefore represent novel targets for the treatment of obesity.

PLoS ONE Alerts: New Articles 2009

Obstacles for using synthetic siRNAs include being a nonrenewable resource, the transient nature of silencing, and the difficulty faced in transfecting primary cells and nondividing cell lines such as neurons, lymphocytes, and macrophages.

GEN News Highlights 2009