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Tasks: Membrane protein expression, purification, cryo-EM imaging, 2D crystallization, cryo-electron crystallography data collection, image processing, 3D structure reconstruction, model building, high-resolution interpretation of the functional consequences of the maps.
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Tasks: Membrane protein expression, purification, cryo-EM imaging, 2D crystallization, cryo-electron crystallography data collection, image processing, 3D structure reconstruction, model building, high-resolution interpretation of the functional consequences of the maps.
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The cryo-EM adenovirus structure is one of only a handful of atomic resolution cryo-EM structures that have been solved to date.
Slashdot: Science 2010
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Experience in at least two of the following techniques is essential: crystallization of membrane proteins in 2D, electron cryo-crystallography, and cryo-EM image analysis.
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The cryo-EM adenovirus structure is one of only a handful of atomic resolution cryo-EM structures that have been solved to date.
Slashdot: Science 2010
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Using a combination of crystallography and cryo-EM, the authors concluded that kinesin-8 motors respond to both MT and tubulin substrates, reflecting their multi-tasking activities.
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Cryo-electron microscopy (cryo-EM) can be used, but the resolution is usually not high enough to see atomic details, because electron irradiation severely damages biological samples.
R&D Mag - News 2010
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"We developed techniques to analyze the structure at high resolution using cryo-EM," explains Yonekura.
R&D Mag - News 2010
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PDBe is the founder of the worldwide archive of 3D cryo-EM data, the Electron Microscopy
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Experience in at least two of the following techniques is essential: crystallization of membrane proteins in 2D, electron cryo-crystallography, and cryo-EM image analysis.
alexz commented on the word cryo-EM
cryo-electron microscopy
October 7, 2017